Graphene nanofiber composites for enhanced neuronal differentiation of human mesenchymal stem cells

Aim: To differentiate mesenchymal stem cells into functional dopaminergic neurons using an electrospun polycaprolactone (PCL) and graphene (G) nanocomposite. Methods: A one-step approach was used to electrospin the PCL nanocomposite, with varying G concentrations, followed by evaluating their biocompatibility neuronal differentiation. Results: exiguous demonstrated ideal nanotopography unprecedented combination of guidance stimuli substrate cues, aiding enhanced differentiation neurons. These newly differentiated were seen exhibit unique arborization, intracellular Ca 2+ influx dopamine secretion. Conclusion: Having cost-effective fabrication room-temperature storage, PCL-G nanocomposites could pave way for differentiation, thereby opening a new horizon array applications in neural regenerative medicine.